OsLSD1, a Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation

OsLSD1, a Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation

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The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD).In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified.The expression of OsLSD1 was light-induced or dark-suppressed.

Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants.Antisense transgenic rice jake wire tighteners plants exhibited lesion mimic phenotype, increased expression of PR-1mRNA, and an accelerated hypersensitive response when inoculated with avirulent isolates of blast fungus.Both sense and antisense transgenic rice plants conferred gemplers free shipping significantly enhanced resistance against a virulent isolate of blast fungus.

Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin.OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells.Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.